A simple, rapid, accurate, specific and sensitive reverse phase liquid chromatographic method (RP-HPLC) was developed and validated for the simultaneous estimation of Rabeprazole and Cinitapride in combined dosage form. The method was standardized using a Sunfire C18 column (250mm×4.6mm ID; Particle size 5μm) and the mobile phase consisted of acetonitrile: mixed phosphate buffer at 50:50%v/v. The eluents were monitored at 277nm and at 1ml/min flow rate. The retention time was found to be 3.966min for Rabeprazole and 5.898min for Cinitapride. The developed method was validated in terms of linearity, accuracy, precision, specificity, limit of detection and limit of quantification, in accordance with the ICH guidelines3. Linearity was obtained with correlation coefficient value r2 = 0.999 for Rabeprazole and r2 = 1 for Cinitapride. The proposed method can be used for the estimation of these drugs in combined dosage form.
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